3CQX

Chaperone Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-08
Detector	NOIR-1
Wavelength(s)	1.07
Spacegroup name	P 21 21 21
Unit cell lengths	52.846, 105.808, 210.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.130 - 2.300
Rwork	0.222
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hx1
RMSD bond length	0.006
RMSD bond angle	1.153
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.130	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.179
Total number of observations		38704
Number of reflections	53515
<I/σ(I)>	18.1	8
Completeness [%]	100.0	100
Redundancy	6.96	7.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K