3CQT
N53I V55L MUTANT of FYN SH3 DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.032, 52.032, 44.817 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.060 - 1.600 |
| R-factor | 0.20881 |
| Rwork | 0.207 |
| R-free | 0.23652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fyn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | COMO |
| Refinement software | REFMAC (5.3.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.060 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.118 |
| Number of reflections | 8962 | |
| <I/σ(I)> | 53.07 | 33.05 |
| Completeness [%] | 93.7 | 97.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Sodium Cacodylate, 1.4 M Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
