3CQS
A 3'-OH, 2',5'-phosphodiester substitution in the hairpin ribozyme active site reveals similarities with protein ribonucleases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.160, 93.160, 129.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.920 - 2.800 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.25400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2p7f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 8458 | |
| <I/σ(I)> | 15.5 | 1.4 |
| Redundancy | 5.09 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20.5% (w/v) PEG 2000 MME, 0.10 M Na cacodylate buffer, 0.25 M Li2SO4, 2.5 mM Co(NH3)6Cl3, 2 mM spermidine-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 MME | ||
| 2 | 1 | 1 | Li2SO4 | ||
| 3 | 1 | 1 | spermidine | ||
| 4 | 1 | 1 | sodium cacodylate | ||
| 5 | 1 | 1 | Co(NH3)6Cl3 | ||
| 6 | 1 | 2 | PEG 2K MME | ||
| 7 | 1 | 2 | Li2SO4 | ||
| 8 | 1 | 2 | HEPES | ||
| 9 | 1 | 2 | spermidine |
