3CQL

Crystal Structure of GH family 19 chitinase from Carica papaya

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-01
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	69.000, 44.490, 76.810
Unit cell angles	90.00, 94.97, 90.00

Refinement procedure

Resolution	19.680 - 1.500
R-factor	0.16226
Rwork	0.161
R-free	0.18679
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.171
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.064	0.325
Number of reflections	71589
<I/σ(I)>		0.064

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	lithium sulfate 0.17M, PEG 4000 25%, glycerol 15%, N-acetyl-D-glucosamine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K