3CQI
Crystal Structure of L-xylulose-5-phosphate 3-epimerase UlaE (form B) complex with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.522, 132.529, 82.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22300 |
| Starting model (for MR) | 3cqh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.185 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.069 | 0.028 | 0.407 |
| Number of reflections | 31993 | ||
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 96.7 | 98.7 | 83.1 |
| Redundancy | 7.1 | 7.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | PEG 8000, Li2SO4, pH 8.5, vapor diffusion, hanging drop, temperature 294K |
