3CQD
Structure of the tetrameric inhibited form of phosphofructokinase-2 from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-30 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.438 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 42.822, 86.834, 171.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.940 - 1.980 |
| R-factor | 0.18881 |
| Rwork | 0.186 |
| R-free | 0.23377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2abq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.483 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.075 | 0.302 |
| Number of reflections | 43478 | |
| <I/σ(I)> | 18.6 | 4.3 |
| Completeness [%] | 93.8 | 90.1 |
| Redundancy | 5.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 0.1 M sodium acetate, 12% PEG 6000, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
