3CQC
Nucleoporin Nup107/Nup133 interaction complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.744, 127.936, 152.738 |
| Unit cell angles | 90.00, 97.36, 90.00 |
Refinement procedure
| Resolution | 39.633 - 2.530 |
| R-factor | 0.2184 |
| Rwork | 0.217 |
| R-free | 0.24550 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.640 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Number of reflections | 32244 | |
| <I/σ(I)> | 16.1 | 2 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M Hepes, 1.1M sodium malonate, 1-1.5% PEG MME 5000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
