3CQB
Crystal structure of heat shock protein HtpX domain from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-11 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.873, 75.873, 65.471 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.370 - 1.860 |
| R-factor | 0.15435 |
| Rwork | 0.152 |
| R-free | 0.19227 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.475 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.095 | 0.847 |
| Number of reflections | 27657 | |
| <I/σ(I)> | 33.8 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.5M Na malonate pH 7.0, 0.1M Tris-HCl pH 7.0, 0.5% Jeffamine ED-2001 pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
