3CQ6
Histidinol-phosphate aminotransferase from Corynebacterium glutamicum holo-form (PLP covalently bound )
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.798, 80.017, 88.484 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 30.790 - 2.100 |
| R-factor | 0.19962 |
| Rwork | 0.198 |
| R-free | 0.23837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cq5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.228 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 75438 | |
| <I/σ(I)> | 10.4 | 1.8 |
| Completeness [%] | 96.9 | 96.8 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.4M NaH2PO4, 1.6M K2HPO4, 0.2M NaCl, 0.1M imidazole, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
