3CQ3
Structure of the DTDP-4-Keto-L-Rhamnose Reductase related protein (other form) from Thermus Thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-14 |
Detector | RIGAKU |
Wavelength(s) | 0.9 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 175.122, 175.122, 99.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.610 - 2.100 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cu6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.064 | 0.280 |
Number of reflections | 82251 | |
Completeness [%] | 99.8 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 18% PEG400, 0.1M Na Acetate, 0.1M Magnesium Chloride , pH4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |