3CQ0
Crystal Structure of TAL2_YEAST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 85.027, 113.461, 158.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f05 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.999 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.073 | 0.022 | 0.396 |
| Number of reflections | 60407 | ||
| <I/σ(I)> | 18.3 | ||
| Completeness [%] | 99.4 | 95.2 | 99.1 |
| Redundancy | 4.9 | 4.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 283 | 1.3M potassium citrate, 10% polyethylene glycol 200, 1% ethylene glycol, 0.1mM Hepes, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
