3CPG
Crystal structure of an unknown protein from Bifidobacterium adolescentis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 48.242, 48.242, 181.319 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.780 - 1.710 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.101 | 0.189 |
| Number of reflections | 25763 | |
| <I/σ(I)> | 21.5 | 10 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 22.5 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M Na acetate, 30% PEG 3350, 0.2 Ammonium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
