3CP2
Crystal structure of GidA from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0438 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 197.600, 197.600, 69.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.960 - 2.900 |
| R-factor | 0.24897 |
| Rwork | 0.246 |
| R-free | 0.31349 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.390 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.054 | 0.267 |
| Number of reflections | 18054 | |
| <I/σ(I)> | 22.85 | 4.9 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 9.4 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM tri-Sodium Citrate pH 5.5, 550mM Ammoniumsulfate, 3% Isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
