3COP
Crystal Structure of E.coli GS mutant E377A in complex with ADP and acceptor analogue HEPPSO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2006-03-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.03 |
Spacegroup name | I 41 |
Unit cell lengths | 125.840, 125.840, 151.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.900 - 2.300 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qzs |
RMSD bond length | 0.009 |
RMSD bond angle | 1.251 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.078 | 0.422 |
Number of reflections | 48231 | |
<I/σ(I)> | 13.7 | 2 |
Completeness [%] | 93.0 | 94.6 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40% (w/v) PEG 4000, 0.1 M HEPPSO, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |