3COP
Crystal Structure of E.coli GS mutant E377A in complex with ADP and acceptor analogue HEPPSO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2006-03-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.03 |
| Spacegroup name | I 41 |
| Unit cell lengths | 125.840, 125.840, 151.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.900 - 2.300 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qzs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.251 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 | 0.422 |
| Number of reflections | 48231 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 93.0 | 94.6 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40% (w/v) PEG 4000, 0.1 M HEPPSO, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
