3COL
Crystal structure of putative transcription regulator from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.213, 66.936, 93.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.770 - 2.100 |
| R-factor | 0.18838 |
| Rwork | 0.185 |
| R-free | 0.24618 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.353 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.105 | 0.444 |
| Number of reflections | 24247 | |
| <I/σ(I)> | 27.27 | 5.71 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 8.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 20 % PEG 3000, 100 mM Na citrate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
