3CO5
Crystal structure of sigma-54 interaction domain of putative transcriptional response regulator from Neisseria gonorrhoeae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 32 |
Unit cell lengths | 67.963, 67.963, 64.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.200 - 2.400 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.24100 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.667 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.200 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.070 | 0.837 |
Number of reflections | 13120 | |
<I/σ(I)> | 12.3 | 2.86 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 11.4 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.17 M Ammonium acetate, 0.085 M Sodium citrate, 25.5% PEG 4000, 15% Glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |