3CNU
Crystal structure of the predicted coding region AF_1534 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-26 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 55.734, 57.614, 68.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.150 - 1.900 |
| R-factor | 0.18501 |
| Rwork | 0.183 |
| R-free | 0.22195 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.150 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.481 |
| Number of reflections | 8550 | |
| <I/σ(I)> | 22.17 | 2 |
| Completeness [%] | 99.5 | 96.49 |
| Redundancy | 7.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 289 | 0.1M Citric acid, 25% PEG 3350, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
