3CNC

Crystal Structure of Ad16 fiber knob

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	167.830, 167.830, 262.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.320 - 2.400
R-factor	0.245
Rwork	0.245
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ad11 fiber knob
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.112	0.881
Number of reflections	85182
<I/σ(I)>	45.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM HEPES, pH 7.5, 0.7M NaH2PO4 and 0.85M KH2PO4, VAPOR DIFFUSION, HANGING DROP, 293K