3CN8
Crystal structure of fms1 in complex with spermidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9779 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.524, 215.458, 119.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.27210 |
Starting model (for MR) | 1yy5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.074 | 0.401 |
Number of reflections | 62677 | |
<I/σ(I)> | 1.4 | |
Completeness [%] | 93.3 | 81.6 |
Redundancy | 5.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 10% PEG8000, 0.2M Li2SO4, 0.1M Tris-HCl, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |