3CN5
Crystal structure of the Spinach Aquaporin SoPIP2;1 S115E, S274E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8726 |
| Spacegroup name | I 4 |
| Unit cell lengths | 87.033, 87.033, 87.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.15996 |
| Rwork | 0.159 |
| R-free | 0.18145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z98 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.293 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 20465 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 280 | 30% PEG400, 0.1M NaCl, 0.1M MgCl2, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
