3CMO
HIV neutralizing monoclonal antibody YZ18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.650, 69.940, 84.360 |
| Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| Rwork | 0.248 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yeh |
| RMSD bond angle | 0.007 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 37445 | |
| <I/σ(I)> | 5.9 | 1.9 |
| Completeness [%] | 95.5 | 95 |
| Redundancy | 3 | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 18% PEG6000, 0.1 M sodium citrate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
