3CM5
Crystal structure of Cell-Death Related Nuclease 4 (CRN-4) bound with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 125.132, 165.432, 64.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.810 |
| R-factor | 0.20314 |
| Rwork | 0.201 |
| R-free | 0.23877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cg7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.810 |
| Number of reflections | 30689 | |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 92.2 | 81 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 5% isopropanal, 0.1M HEPES pH 7.5, 10% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
