3CM1

Crystal structure of SsgA-like sporulation-specific cell division protein (YP_290167.1) from Thermobifida fusca YX-ER1 at 2.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97929, 0.97898
Spacegroup name	P 41
Unit cell lengths	64.840, 64.840, 130.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.029 - 2.600
R-factor	0.232
Rwork	0.230
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.414
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.029	46.029	2.690
High resolution limit [Å]	2.600	5.570	2.600
Rmerge	0.046	0.027	0.793
Number of reflections	16529	1666	1579
<I/σ(I)>	15.84	36.5	1.8
Completeness [%]	99.4	96.4	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	NANODROP, 40.0% 1,2-propanediol, 0.1M Acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K