3CLW
Crystal structure of conserved exported protein from Bacteroides fragilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.530, 116.258, 393.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.199 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.412 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.199 | 2.199 |
| Rmerge | 0.101 | 0.551 |
| Total number of observations | 129542 | |
| Number of reflections | 172557 | |
| <I/σ(I)> | 14.8 | 3 |
| Completeness [%] | 84.5 | 79.5 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 200mM Sodium sulfate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, temperature 294K |
