3CLR
Crystal structure of the R236A ETF mutant from M. methylotrophus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 |
| Unit cell lengths | 117.009, 117.009, 84.513 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.14947 |
| Rwork | 0.148 |
| R-free | 0.18169 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o97 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.557 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 49299 |
| <I/σ(I)> | 8.6 |
| Completeness [%] | 99.8 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | limited digest with trypsin, 2.0 M sodium phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP |
