3CLE
HIV neutralizing monoclonal antibody YZ23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.290, 62.919, 135.172 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kn2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.220 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15906 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 97.0 | 99 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 16% PEG6000, 0.1 M HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
