3CKU
Urate oxidase from aspergillus flavus complexed with its inhibitor 8-azaxanthin and chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.964 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.994, 94.979, 104.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.197 |
| Rwork | 0.193 |
| R-free | 0.25100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r4s |
| RMSD bond length | 0.033 |
| RMSD bond angle | 0.077 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | FFT |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.056 | 0.099 |
| Number of reflections | 42048 | |
| <I/σ(I)> | 22 | 13.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2MG/ML 8-AZAXANTHIN, 0.3M SODIUM CHLORIDE, 0.05M TRIS/HCL, 10-15% W/V PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
