3CKO

Crystal Structure of a Mycobacterial Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-08-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	86.823, 86.823, 103.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.300 - 2.500
R-factor	0.22044
Rwork	0.220
R-free	0.23035
Structure solution method	Rigid body refinement
RMSD bond length	0.005
RMSD bond angle	0.887
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.300
High resolution limit [Å]	2.500	2.500
Number of reflections	13439
<I/σ(I)>	9.3
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	1 M Ammonium sulphate, 0.1 M Sodium citrate pH 5.5, 20 mM Manganese chloride, 1 mM Sodium thiosulphate, 100 mM uridine-5 -diphosphate-glucose , VAPOR DIFFUSION, HANGING DROP, temperature 293K