3CK6
Crystal structure of ZntB cytoplasmic domain from Vibrio parahaemolyticus RIMD 2210633
Replaces: 3BHCExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-15 |
Detector | SBC-3 |
Wavelength(s) | 1.28234 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.390, 130.213, 79.386 |
Unit cell angles | 90.00, 103.41, 90.00 |
Refinement procedure
Resolution | 30.800 - 1.900 |
R-factor | 0.18689 |
Rwork | 0.185 |
R-free | 0.22347 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.284 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.800 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.104 | 0.849 |
Number of reflections | 97781 | |
<I/σ(I)> | 23.17 | 1.22 |
Completeness [%] | 98.1 | 81.8 |
Redundancy | 6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M MgCl2, 0.1M Tris-HCl, 30% PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |