3CK4
A heterospecific leucine zipper tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.846, 49.107, 51.917 |
| Unit cell angles | 115.77, 94.21, 109.62 |
Refinement procedure
| Resolution | 45.180 - 1.700 |
| R-factor | 0.17305 |
| Rwork | 0.171 |
| R-free | 0.22062 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b22 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.537 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.042 | 0.413 |
| Number of reflections | 41918 | |
| <I/σ(I)> | 12.7 | 2.1 |
| Completeness [%] | 96.5 | 95.8 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris-HCl, 0.2M magnesium chloride, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
