3CJS
Minimal Recognition Complex between PrmA and Ribosomal Protein L11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.211, 33.948, 71.090 |
| Unit cell angles | 90.00, 131.76, 90.00 |
Refinement procedure
| Resolution | 25.720 - 1.370 |
| R-factor | 0.18166 |
| Rwork | 0.180 |
| R-free | 0.20601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 2NXC 2nxn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.420 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.053 | 0.386 |
| Number of reflections | 37200 | |
| <I/σ(I)> | 22.5 | 2.6 |
| Completeness [%] | 95.1 | 84.7 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 22% w/v PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
