3CJN
Crystal structure of transcriptional regulator, MarR family, from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 87.976, 87.976, 43.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.980 - 1.950 |
| R-factor | 0.18119 |
| Rwork | 0.179 |
| R-free | 0.22257 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.282 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.094 | 0.472 |
| Number of reflections | 12941 | |
| <I/σ(I)> | 77.2 | 14.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21 | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281 | 0.1 Succinic acid pH 7.0, 12 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 281K |
