3CJB
Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.696, 69.540, 182.106 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.910 - 3.210 |
R-factor | 0.246 |
Rwork | 0.245 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.014 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 120.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.236 | 0.418 |
Number of reflections | 9573 | |
<I/σ(I)> | 3.6 | |
Completeness [%] | 77.5 | 50.2 |
Redundancy | 2.7 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% Peg MME 5000, 5% Tacsimate, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |