3CJ4
Crystal structure of hepatitis c virus rna-dependent rna polymerase ns5b in complex with optimized small molecule fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.143, 86.259, 116.141 |
| Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
| Resolution | ? - 2.070 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.27800 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 69.210 |
| High resolution limit [Å] | 2.070 |
| Number of reflections | 69870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.9 | 292 | 8-11% GLYCEROL, 20-24% PEG 4000,50 mM Sodium CITRATE, pH 4.9, VAPOR DIFFUSION, temperature 292K |
