3CJ1
Structure of Rattus norvegicus NTPDase2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.346, 69.029, 164.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.780 - 1.700 |
| R-factor | 0.17567 |
| Rwork | 0.175 |
| R-free | 0.20385 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.430 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.480 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.078 | 0.513 |
| Number of reflections | 52974 | |
| <I/σ(I)> | 13.7 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 100mM NaHEPES, 2% PEG 6000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
