3CIT
Crystal structure of the GAF domain of a putative sensor histidine kinase from Pseudomonas syringae pv. tomato
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9793, 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.471, 107.471, 67.698 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.220 - 1.900 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20000 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.424 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.507 |
| Number of reflections | 35808 | |
| <I/σ(I)> | 10.9 | 4 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 11 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M Bis-Tris propane pH 7.0, 1.5M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
