3CI6
Crystal structure of the GAF domain from Acinetobacter phosphoenolpyruvate-protein phosphotransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931, 0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.081, 54.992, 124.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.170 - 1.550 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19600 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.538 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.095 | 0.501 |
| Number of reflections | 39536 | |
| <I/σ(I)> | 9 | 3.9 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 10.3 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris-HCl pH 8.5, 0.2M MgCl2, 30% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
