3CI5
Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.423, 69.045, 56.540 |
| Unit cell angles | 90.00, 104.20, 90.00 |
Refinement procedure
| Resolution | 38.120 - 1.700 |
| R-factor | 0.14339 |
| Rwork | 0.141 |
| R-free | 0.19087 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nm1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.365 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.084 | 0.407 |
| Number of reflections | 69701 | |
| <I/σ(I)> | 23.1 | 2.2 |
| Completeness [%] | 94.9 | 57.9 |
| Redundancy | 8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 10% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
