3CHK
Calcium bound structure of alpha-14 giardin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | CCD |
| Collection date | 2007-04-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.301, 50.018, 71.819 |
| Unit cell angles | 90.00, 110.04, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.23700 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.650 |
| Number of reflections | 38460 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 50 mM calcium chloride, 100 mM bis-Tris pH 6.5, and 24% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
