3CGW

Crystal structure of 2-phospho-(S)-lactate transferase from Methanosarcina mazei. Northeast Structural Genomics Consortium target MaR46

Replaces:  2FFE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97933, 0.97947, 0.96771
Spacegroup name	P 43 21 2
Unit cell lengths	110.640, 110.640, 75.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 3.100
R-factor	0.239
Rwork	0.239
R-free	0.27700
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.450	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.134	0.522
Number of reflections	16408
<I/σ(I)>	17.88	2.26
Completeness [%]	99.2	94.7
Redundancy	9.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM Sodium chloride, 5 mM DTT. Reservoir solution: 16% PEG 3350 and 200 mM LiNO3, VAPOR DIFFUSION, HANGING DROP, temperature 291K