3CGQ
X-ray structure of a pseudouridine-containing yeast spliceosomal U2 snRNA-intron branch site duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-21 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.700, 41.010, 32.970 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.1986 |
| Rwork | 0.193 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Combination of PDB entry 1I9X and 1NUV |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.550 |
| Number of reflections | 3027 | |
| <I/σ(I)> | 44.1 | 16.5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 2 | ammonium sulfate |
