3CGN
Crystal Structure of thermophilic SlyD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2007-11-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91840 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 81.180, 85.090, 92.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.700 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cgm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.048 | 0.472 |
| Number of reflections | 4147 | 359 |
| <I/σ(I)> | 16.83 | 2.8 |
| Completeness [%] | 90.6 | 78.2 |
| Redundancy | 5.13 | 5.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 286 | 100mM MES, pH6.5, 1.6M Na/K-Tartrate, 26mg/ml, 1:1 mixing, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
