3CGM
Crystal structure of thermophilic SlyD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 121.780, 121.780, 76.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.270 - 2.410 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.22600 |
| Structure solution method | MR |
| Starting model (for MR) | 1d6o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.550 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.093 | 0.582 |
| Number of reflections | 12921 | 1885 |
| <I/σ(I)> | 21.98 | 4.4 |
| Completeness [%] | 96.6 | 92.9 |
| Redundancy | 13.1 | 11.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 286 | 60mg/ml protein, 1:1 mixing, 2M Ammonium sulfate, 2% PEG 400, 1% Thiocyanate, 100mM HEPES, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
