3CG8
Laccase from Streptomyces coelicolor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.3775, 1.380, 0.954 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 180.868, 180.868, 177.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.540 - 2.679 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.19400 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.558 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (C) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.095 | 0.640 |
| Number of reflections | 80263 | |
| <I/σ(I)> | 11.6 | 9.4 |
| Completeness [%] | 94.1 | 70.6 |
| Redundancy | 17 | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 39 % (v/v) PEG monomethyl ether 550, 0.1 M NaCl, 0.1 M glycine, pH 9.0; 2:1 protein:reservoir ratio in a drop; data from this crystal were used for structure refinement, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
