3CG6
Crystal structure of Gadd45 gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.024, 122.468, 105.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.100 - 1.700 |
| R-factor | 0.22617 |
| Rwork | 0.224 |
| R-free | 0.27169 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.450 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.100 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 | 0.269 |
| Number of reflections | 30674 | |
| <I/σ(I)> | 15.7 | 3.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.74 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, 1.9 M NaH2PO4 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
