3CFU
Crystal structure of the yjhA protein from Bacillus subtilis. Northeast Structural Genomics Consortium target SR562
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.678, 41.687, 65.656 |
| Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
| Resolution | 25.780 - 2.400 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.255 |
| Number of reflections | 25820 | |
| <I/σ(I)> | 18.53 | 3.8 |
| Completeness [%] | 97.7 | 92.2 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7 | 277 | 40% PEG 4000, 0.1M Lithium sulfate, 0.1M MOPS pH 7.0, MICROBATCH UNDER OIL, temperature 277K |
