3CFF
Photoswitchable red fluorescent protein psRFP, on-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.418, 98.360, 242.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.270 - 1.800 |
| R-factor | 0.19753 |
| Rwork | 0.196 |
| R-free | 0.22401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cfa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.095 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.073 | 0.580 |
| Number of reflections | 102342 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 97.0 | 95.6 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 0.2M di-Sodium tartate, 40%(w/v) MPD, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
