3CFE
Crystal structure of purple-fluorescent antibody EP2-25C10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.130, 59.460, 149.370 |
| Unit cell angles | 90.00, 100.37, 90.00 |
Refinement procedure
| Resolution | 24.460 - 2.990 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.25500 |
| Structure solution method | PHASER |
| Starting model (for MR) | 3hfm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.070 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 20613 | |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 99.3 | 89.5 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | AMMONIUM SULFATE, LITHIUM SULFATE, ACETATE, PH 4.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 4.50 |
