3CEX
Crystal structure of the conserved protein of locus EF_3021 from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 55.191, 55.137, 62.429 |
| Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
| Resolution | 35.900 - 2.000 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20500 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.373 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.107 | 0.410 |
| Number of reflections | 23947 | |
| <I/σ(I)> | 6.6 | 2.4 |
| Completeness [%] | 98.9 | 89.32 |
| Redundancy | 6.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1M Trisodium citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
