3CES

Crystal Structure of E.coli MnmG (GidA), a Highly-Conserved tRNA Modifying Enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-28
Wavelength(s)	0.9798
Spacegroup name	P 1 21 1
Unit cell lengths	85.904, 144.326, 147.745
Unit cell angles	90.00, 106.63, 90.00

Refinement procedure

Resolution	141.420 - 2.412
R-factor	0.203
Rwork	0.201
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.534
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	141.420
High resolution limit [Å]	2.412
Rmerge	0.115
Number of reflections	129226
<I/σ(I)>	11.6
Completeness [%]	99.6
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	PEG 8000, Sodium Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	PEG 8000, Sodium Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K